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<p><b>OBJECTIVE</b>To evaluate the effect of continuous blood purification (CBP) for treatment of multiple organ failure (MOF) with acute renal failure (ARF).</p><p><b>METHODS</b>Forty-seven patients with MOF underwent CBP for an average of 3.1-/+0.5 days averagely. Continuous veno-venous hemofiltration was performed at daytime, and the substitute fluids were infused with pre-dilution at the rate of 2000-4000 ml/h. The general conditions, clinical symptoms, and serum biochemical indexes of the patients were observed and MODS score was calculated.</p><p><b>RESULTS</b>After CBP, the MODS score of the patients decreased significantly from 9.1-/+3.5 to 5.4-/+2.7 (P<0.01) and serum creatinine decreased from 451.3-/+134.5 to 223.7-/+100.2 micromol/L (P<0.05). Twenty-nine patients survived with the survival rate of 61.7%.</p><p><b>CONCLUSION</b>CBP is effective for treatment of MOF and may help reduce the mortality rate of MOF complicated by ARF.</p>
Subject(s)
Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Young Adult , Hemofiltration , Methods , Multiple Organ Failure , Therapeutics , Pancreatitis , Renal Insufficiency , Therapeutics , Treatment Outcome , Wounds and InjuriesABSTRACT
<p><b>OBJECTIVE</b>To investigate, at the DNA level, the polymorphism of HLA-A, -B, -Cw genes in the Chinese of Han ethnicity in Shenyang.</p><p><b>METHODS</b>Hybridization with polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP) was used to determine HLA-A, -B and -Cw genotypes of 108 unrelated healthy individuals from a Chinese Han population. These Hans were born and living in the Shenyang area.</p><p><b>RESULTS</b>The numbers of alleles identified were 21 for HLA-A, 43 for HLA-B, and 23 for HLA-Cw. All the allele frequency distributions were consistent with the Hardy-Weinberg equilibrium.</p><p><b>CONCLUSION</b>Using molecular method, the present authors have analyzed the characteristic of HLA I distribution in a group of indigenous Hans in Shenyang and thus have provided more accurate gene data for use in related researches.</p>
Subject(s)
Adolescent , Adult , Female , Humans , Male , Middle Aged , Young Adult , Alleles , China , DNA , Genetics , Gene Frequency , Genotype , HLA-A Antigens , Genetics , HLA-B Antigens , Genetics , HLA-C Antigens , Genetics , Linkage Disequilibrium , Oligonucleotide Probes , Polymerase Chain Reaction , MethodsABSTRACT
Objective To investigate the expression of glucocorticoid receptor-alpha and beta(GR?and GR?)mRNA of peripheral blood mononuclear cells(PBMCs)in patients with dermatomyositis/polymyositis (DM/PM)and analyze their association with the pathogenesis and clinical response to glucocorticoid therapy. Methods The expression of GR?and GR?mRNA in PBMCs from 20 normal controls and 36(24 steroid- sensitive,12 steroid-resistant)patients with DM/PM were detected by reverse transcription polymerase chain reaction(RT-PCR).Results Compared to normal controls,the expression of GR?mRNA in the DM/PM was significantly decreased(P
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0.05).But GR number[sites/cell]and the expression of GR mRNA in PBMCs from PM/DM was significantly lower than those in healthy controls(P